Martiniello R, Burton R C, Smart Y C
Discipline of Surgical Science, Faculty of Medicine and Health Science, The University of Newcastle, N.S.W., Australia.
Int J Cancer. 1996 May 16;66(4):532-7. doi: 10.1002/(SICI)1097-0215(19960516)66:4<532::AID-IJC18>3.0.CO;2-9.
We have previously shown that anti-Ly-6C monoclonal antibody (MAb) 2B6-F2 identifies a subset of (CBA +/- C57BL/6)F1 splenic NK-1.1+ natural T (NT) cells which kill the NK-sensitive YAC-1 target in vitro. Furthermore, these Ly-6C+ cells are responsible for 40-50% of in vitro YAC-1 killing in all mouse strains tested. In BALB/c and DBA/2 mice, these cells killed not only YAC-1 but also the NK-resistant WEHI-164 M1/16 target via a receptor that recognises a shared determinant on these targets in vitro. In the present study, the anti-tumour role of Ly-6C+ cells against the NK-sensitive B16 melanoma and NK-resistant tumours WEHI-7 T lymphoma and WEHI-164/1C fibrosarcoma was studied in vitro and in vivo. In vitro, B16, WEHI-7 and WEHI-164/1C tumour cell lines were highly sensitive to Ly-6C+ cell killing. In vivo, these same tumours showed significantly increased growth when transplanted s.c. into syngeneic mice treated with 2B6-F2 (0.05 < or = p < 0.0005), and this was most marked in the first 15 days following tumour appearance, when tumours were <15 mm in diameter. Our results show that Ly-6C+ cells play a role in controlling the growth of transplantable NK-sensitive B16 melanoma, and in BALB/c mice, at least, the repertoire of susceptible tumours is extended to include NK-resistant WEHI-7 and WEHI-164/1C. We conclude that Ly-6C+ NT cells play a role in immunosurveillance against NK-sensitive as well as NK-resistant tumours in certain strains of mice.
我们之前已经表明，抗Ly-6C单克隆抗体（MAb）2B6-F2可识别（CBA +/- C57BL/6）F1脾内NK-1.1+天然T（NT）细胞的一个亚群，这些细胞在体外可杀伤NK敏感的YAC-1靶细胞。此外，在所有测试的小鼠品系中，这些Ly-6C+细胞在体外对YAC-1的杀伤作用中占40%-至50%。在BALB/c和DBA/2小鼠中，这些细胞不仅能杀伤YAC-1，还能通过一种识别这些靶细胞上共同决定簇的受体，在体外杀伤NK抗性的WEHI-164 M1/16靶细胞。在本研究中，我们在体外和体内研究了Ly-6C+细胞对NK敏感的B16黑色素瘤以及NK抗性肿瘤WEHI-7 T淋巴瘤和WEHI-164/1C纤维肉瘤的抗肿瘤作用。在体外，B16、WEHI-7和WEHI-164/1C肿瘤细胞系对Ly-6C+细胞的杀伤高度敏感。在体内，当将这些相同的肿瘤皮下移植到用2B6-F2处理的同基因小鼠中时，肿瘤生长显著加快（0.05≤p＜0.0005），且在肿瘤出现后的前15天最为明显，此时肿瘤直径＜15毫米。我们的结果表明，Ly-6C+细胞在控制可移植的NK敏感B16黑色素瘤的生长中发挥作用，并且至少在BALB/c小鼠中，易感肿瘤的范围扩展到包括NK抗性的WEHI-7和WEHI-164/1C。我们得出结论，Ly-6C+ NT细胞在某些品系小鼠中对NK敏感和NK抗性肿瘤的免疫监视中发挥作用。
