Pupyshev A B, Malygin A E, Korolenko T A
Biokhimiia. 1981 Jul;46(7):1167-74.
The effect of suramin on the state of rat liver lysosomal apparatus 24 and 48 hrs after intraperitoneal injection of the drug was studied. The specific activity of liver acid phosphatase was decreased by suramin down to 35-45% of the control. The inhibitory effect of suramin on the activity of acid phosphatase was observed in all subcellular fractions of the liver and was correlated with the relative content of lysosomal enzymes activity. The sedimentability of lysosomes upon distribution among subcellular fractions was increased. The inhibitory effect of suramin on intralysosomal digestion of 14C-labelled bovine serum albumin was found in nuclear and light mitochondrial fractions, but not in the heavy mitochondrial fraction. The functional heterogeneity of the subpopulations of heterolysosomes and the mechanisms of suramin action on intralysosomal proteolysis are discussed.
