[Role of lysosome permeability disorders in the mechanism of the low-temperature inhibition of protein biosynthesis in the rat liver].

The effect of the supernatants of lysosomes subjected to a rapid or slow freezing and thawing on the protein-synthesizing activity of the rat liver postmitochondrial supernatant (S15) has been studied. It has been found that the addition of supernatants obtained after a slow freezing or thawing to a cell-free system results in a significant inhibition of 14C-leucine incorporation that is in a good agreement with their RNase activity. However, the RNase activity of lysosomal supernatants or Triton-lysate appears to be higher that the inhibitory effect on protein biosynthesis. The damaging effect of supernatants obtained after 2-hour storage at 2-4 degrees C of the frozen-thawed lysosomes has not virtually changed.