Gulevskiĭ A K, Zagnoĭko V I, Lugovoĭ V I, Belous A M
Biull Eksp Biol Med. 1981 Jun;91(6):675-6.
The effect of the supernatants of lysosomes subjected to a rapid or slow freezing and thawing on the protein-synthesizing activity of the rat liver postmitochondrial supernatant (S15) has been studied. It has been found that the addition of supernatants obtained after a slow freezing or thawing to a cell-free system results in a significant inhibition of 14C-leucine incorporation that is in a good agreement with their RNase activity. However, the RNase activity of lysosomal supernatants or Triton-lysate appears to be higher that the inhibitory effect on protein biosynthesis. The damaging effect of supernatants obtained after 2-hour storage at 2-4 degrees C of the frozen-thawed lysosomes has not virtually changed.
研究了经快速或缓慢冻融的溶酶体上清液对大鼠肝脏线粒体后上清液（S15）蛋白质合成活性的影响。发现将缓慢冻融后获得的上清液添加到无细胞系统中会导致14C-亮氨酸掺入的显著抑制，这与其核糖核酸酶活性高度一致。然而，溶酶体上清液或曲拉通裂解物的核糖核酸酶活性似乎高于对蛋白质生物合成的抑制作用。冻融溶酶体在2-4℃储存2小时后获得的上清液的损伤作用实际上并未改变。
