Determination of mutagens, amino-alpha-carbolines in grilled foods and cigarette smoke condensate.

The amounts of 2 mutagens, 2-amino-9H-pyrido[2,3-b]indole (AC) and 2-amino-3-methyl-9H-pyrido[2,3-b]indole (MeAC), in grilled foods and cigarette smoke condensate wer3e determined by analysis system composed of HPLC and spectrofluorometer. The AC and MeAC contents of 1 g of grilled beef were 650.8 ng and 63.5 ng, respectively. They were equivalent to those of about 8 cigarettes. The smoke condensate of a blended cigarette (A) contained 79.7 ng of AC and 6.2 ng of MeAC.