Landa V
Folia Biol (Praha). 1981;27(4):265-73.
Rabbit embryos at the 8-cell and morula stages were frozen and stored at -196 degrees C for 2-200 days. After thawing the embryos were examined for their viability in vitro and in vivo. In vitro, 62.5% of frozen 8-cell embryos and 81.4% of frozen morulae developed to blastocysts. In the control group of unfrozen embryos, 93.2% 8-cell embryos and 92.4% morulae developed to the blastocyst stage. Culture permitted a more reliable elimination of the embryos damaged during freezing and thawing. Embryos were transferred into the reproductive tracts of the recipients either directly after thawing or after 24 h in culture. Synchronous transfers of frozen rabbit embryos were not successful. After asynchronous transfers of morulae and blastocysts into the oviducts, implantation was 31.8% and 42.9%, respectively. After transfer of blastocysts into the uterine horns of the recipients, 47.6% embryos implanted.
将处于8细胞期和桑椹胚期的兔胚胎冷冻，并在-196℃下保存2至200天。解冻后，对胚胎进行体外和体内活力检测。体外实验中，62.5%的冷冻8细胞胚胎和81.4%的冷冻桑椹胚发育成囊胚。在未冷冻胚胎的对照组中，93.2%的8细胞胚胎和92.4%的桑椹胚发育到囊胚阶段。培养有助于更可靠地淘汰在冷冻和解冻过程中受损的胚胎。胚胎在解冻后或培养24小时后直接移植到受体的生殖道中。冷冻兔胚胎的同步移植未成功。将桑椹胚和囊胚异步移植到输卵管后，着床率分别为31.8%和42.9%。将囊胚移植到受体的子宫角后，47.6%的胚胎着床。
