Szydłowska H, Kowalska Z, Vetulani J
Folia Histochem Cytochem (Krakow). 1981;19(2):93-8.
Explants of nervous tissue taken up from the area of locus coeruleus from rats 3-36 days old were cultured in vitro for 15 days, and than accumulation of norepinephrine (3 X 10-8M) was studied with and without preincubation with equimolar concentration of clonidine, using histofluorescence methods. Preincubation with clonidine increased the fluorescence in neural cells, particularly those derived from young animals, in which the pattern of fluorescence appeared to resemble the observed without preincubation only in cells from older animals in addition to fluorescence in the cells, fluorescent granules appeared in neuronal processes and in structures resembling non adrenergic nerve endings. Clonidine completely inhibited norepinephrine uptake by glial cells from very young animals, and inhibited that in the glia in colonies derived from 18-24 days old rats. However, clonidine preincubation restored accumulation of norepinephrine by oligodendroglia from 36 days old rats already lost by this age, but did not affect astrocytes.
从3至36日龄大鼠的蓝斑区域取出神经组织外植体，在体外培养15天，然后使用组织荧光法研究在有无等摩尔浓度可乐定预孵育的情况下去甲肾上腺素（3×10⁻⁸M）的积累情况。可乐定预孵育增加了神经细胞中的荧光，特别是来自幼龄动物的神经细胞，其中荧光模式似乎类似于仅在来自老龄动物的细胞中未进行预孵育时观察到的情况，除了细胞中的荧光外，在神经元突起和类似非肾上腺素能神经末梢的结构中也出现了荧光颗粒。可乐定完全抑制了极幼龄动物神经胶质细胞对去甲肾上腺素的摄取，并抑制了来自18至24日龄大鼠的集落中神经胶质细胞对去甲肾上腺素的摄取。然而，可乐定预孵育恢复了36日龄大鼠少突胶质细胞对去甲肾上腺素的积累，该积累在此年龄时已经丧失，但对星形胶质细胞没有影响。
