Lobb C J, Clem L W
J Immunol. 1981 Oct;127(4):1525-9.
Passive administration of homologous, radioiodinated, purified high m.w. (HMW) or low m.w. (LMW) serum immunoglobulin (Ig) into the sheepshead, a marine teleost, showed that the LMW Ig was neither an extracellular precursor nor a degradation product of the HMW Ig. These studies also showed that the half-lives of the HMW and LMW Ig were statistically identical, i.e., approximately 16 days. Furthermore, this work demonstrated that the Ig found in cutaneous mucus and bile were not due to transudation or active transport of either the serum HMW or LMW Ig. The implications of these later findings, coupled with previous physicochemical characterizations of the Ig in fish secretions, support the hypothesis that fish, similar to higher vertebrates, have a secretory immune system.
将同源的、经放射性碘化的、纯化的高分子量（HMW）或低分子量（LMW）血清免疫球蛋白（Ig）被动注入羊头鱼（一种海洋硬骨鱼）体内，结果表明LMW Ig既不是HMW Ig的细胞外前体，也不是其降解产物。这些研究还表明，HMW和LMW Ig的半衰期在统计学上是相同的，即大约16天。此外，这项工作证明，皮肤黏液和胆汁中发现的Ig并非血清HMW或LMW Ig的渗漏或主动转运所致。这些最新发现的意义，再加上之前对鱼类分泌物中Ig的物理化学特性描述，支持了这样一种假说，即鱼类与高等脊椎动物类似，拥有一个分泌免疫系统。
