Hussain R, Hamilton R G, Kumaraswami V, Adkinson N F, Ottesen E A
J Immunol. 1981 Oct;127(4):1623-9.
We have developed a noncompetitive solid phase radioimmunoassay to quantitate human IgE antibodies against soluble adult antigens of Brugia malayi (B.m.), a filarial parasite, in sera of patients with various forms of clinical filariasis in Madras, India. A single reference serum was shown to contain 23 micrograms/ml of B.m.-specific IgE by depletion analysis and was used as a standard serum throughout the study. The levels of specific IgE ranged in the patients sera from 2 to 23,000 ng/ml. When these individuals were divided into clinical groups, the individuals with tropical pulmonary eosinophilia had the highest levels (mean = 8630 ng/ml) and were significantly higher than all the other groups (p less than 0.001). The lowest levels were seen in patients with circulating microfilariae (mean = 30.5 ng/ml). Patients exhibiting lymphatic obstruction (i.e., chronic pathology group) had levels slightly higher than microfilaremics (mean = 68 ng/ml) but were not significantly different (p less than 0.1). Surprisingly, individuals living in endemic areas but who had no clinical signs of filariasis also showed appreciable levels of B.m.-specific IgE (mean = 55 ng/ml). The B.m.-specific IgE represented 0.1 to 48% of the total IgE. High percentages of specific IgE may be responsible for evoking allergic symptomatology in patients with tropical pulmonary eosinophilia.
我们开发了一种非竞争性固相放射免疫测定法，用于定量印度马德拉斯患有各种临床丝虫病的患者血清中针对马来布鲁线虫（B.m.）可溶性成虫抗原的人IgE抗体。通过耗竭分析显示，单一参考血清含有23微克/毫升的B.m.特异性IgE，并在整个研究中用作标准血清。患者血清中特异性IgE水平在2至23,000纳克/毫升之间。当将这些个体分为临床组时，热带肺嗜酸性粒细胞增多症患者的水平最高（平均值 = 8630纳克/毫升），且显著高于所有其他组（p < 0.001）。循环微丝蚴患者的水平最低（平均值 = 30.5纳克/毫升）。表现出淋巴阻塞的患者（即慢性病理组）的水平略高于微丝蚴血症患者（平均值 = 68纳克/毫升），但差异不显著（p < 0.1）。令人惊讶的是，生活在流行地区但无丝虫病临床症状的个体也显示出相当水平的B.m.特异性IgE（平均值 = 55纳克/毫升）。B.m.特异性IgE占总IgE的0.1%至48%。高百分比的特异性IgE可能是导致热带肺嗜酸性粒细胞增多症患者出现过敏症状的原因。
