Noguchi K
Nihon Sanka Fujinka Gakkai Zasshi. 1981 Aug;33(8):1137-46.
Endometrial hyperplasia was studied from the point of view of cell surface charge employing micro electrophoresis. Surface change of cells corresponding to the cyclic changes of cell multiplication as well as estrogen and/or progesterone effects on the cellular surface charges on each phase of the cell cycle. The results as follows: 1. Electrophoretic mobility was highest in mitotic phase. This result was indicative of what the cell-surface charge was closely related to cell multiplication. 2. Estrogen clearly elevated electrophoretic mobility of cells in synthetic phase. Progesterone lowered electrophoretic mobility of cells in G1 phase. These results indicate that multiplication was forced in synthetic phase by estrogen and differentiation in G1 phase by progesterone. 3. Effects of estrogen and progesterone to cultured pulmonary cancer cells were studied as control to endometrial ones which might be target to these hormones. No definite results as previously shown on endometrial cells were obtained on the pulmonary ones. As a result, it is apparent that endometrium is a target to these hormones. Determination of the cellular surface charge by cell micro electrophoresis indicated that estrogen might play an important role in triggering abnormal proliferations of the endometrium.
采用微电泳技术从细胞表面电荷的角度对子宫内膜增生进行了研究。细胞表面电荷的变化与细胞增殖的周期性变化相对应，同时也与雌激素和/或孕激素对细胞周期各阶段细胞表面电荷的影响有关。结果如下：1. 有丝分裂期的电泳迁移率最高。这一结果表明细胞表面电荷与细胞增殖密切相关。2. 雌激素明显提高了合成期细胞的电泳迁移率。孕激素降低了G1期细胞的电泳迁移率。这些结果表明，雌激素在合成期促使细胞增殖，而孕激素在G1期促使细胞分化。3. 作为子宫内膜细胞可能的激素靶点，研究了雌激素和孕激素对培养的肺癌细胞的影响，并与子宫内膜细胞进行对照。在肺癌细胞上未获得如先前在子宫内膜细胞上所示的明确结果。因此，很明显子宫内膜是这些激素的靶点。通过细胞微电泳测定细胞表面电荷表明，雌激素可能在引发子宫内膜异常增殖中起重要作用。
