Rakonczay Z, Vincendon G, Zanetta J P
J Neurochem. 1981 Sep;37(3):662-9. doi: 10.1111/j.1471-4159.1982.tb12538.x.
According to their solubilization properties, two classes of acetylcholinesterases (AChE) can be detected in the adult rat brain: a "soluble" species (easily solubilized without detergent), and a membrane-bound species (solubilized only in the presence of detergent). The latter was found to be homogeneous by gel filtration (Stokes radius 8.05 +/- 0.35 nm) and sucrose gradient centrifugation (9.75 +/- 0.2 S) in the presence of Triton X-100. The "soluble" AChE gives three stable species in the presence of the same detergent with Stokes radii and sedimentation constants of 10.9 +/- 0.5 nm and 16 +/- 2S; 6.75 +/- 0.30 nm, and 10.7 +/- 0.4 S; 5.37 +/- 0.35 nm and 4.37 +/- 0.1 S. Co-chromatography and co-sedimentation or the reduction and alkylation of disulfide bridges show that all the soluble species are different from the membrane-bound AChE. The possibility that soluble and membrane-bound AChE are completely different molecules is discussed.
根据其溶解特性，在成年大鼠脑中可检测到两类乙酰胆碱酯酶（AChE）：一种“可溶性”类型（无需去污剂即可轻松溶解），以及一种膜结合类型（仅在有去污剂存在时才能溶解）。在Triton X-100存在的情况下，通过凝胶过滤（斯托克斯半径8.05±0.35 nm）和蔗糖梯度离心（9.75±0.2 S）发现后者是均一的。在相同去污剂存在的情况下，“可溶性”AChE产生三种稳定类型，其斯托克斯半径和沉降常数分别为10.9±0.5 nm和16±2 S；6.75±0.30 nm和10.7±0.4 S；5.37±0.35 nm和4.37±0.1 S。共色谱和共沉降或二硫键的还原和烷基化表明，所有可溶性类型均与膜结合的AChE不同。文中讨论了可溶性和膜结合的AChE完全不同分子的可能性。
