Tuli S M, Gupta K B
J Trauma. 1981 Oct;21(10):894-8. doi: 10.1097/00005373-198110000-00011.
A circumferential complete osteoperiosteal gap (1 to 1.5 cm) was produced in the diaphysis of ulna of rabbits, and the ipsilateral radius was kept intact. In no case did spontaneous bridging of the gap occur. By 6 weeks after the creation of the gap the bone ends became smooth and tapered towards the ipsilateral radius and the defect was observed to be filled by thin fibrous tissue. Established defects at least 6 weeks old were then bridged by inserting a snugly fitting allogeneic bone matrix implant. The bone matrix was prepared by demineralization of tubular bones from healthy rabbits, using 0.6 M HCl, preserved in 75% alcohol and used within 3 weeks. The fate of the implant was observed clinically, radiologically, histologically by tetracycline fluorescence for up to 12 weeks. Radiologic evidence of bone formation was present in a majority by 6 weeks, and complete bridging of the gap was seen in 75% of cases by 12 weeks after implantation. Most of the initial bone that formed was the woven bone, i.e., immature bone formed directly from the mesenchymal cells, or formed in membrane, though areas of enchondral ossification were also observed in some cases. By 12 weeks, the bone had remodeled almost to the texture of a mature tubular bone. Successful bridging of chronic massive bone defects by allogeneic bone-matrix was confirmed in 75% of cases.
在兔尺骨干中制造一个环形全骨膜间隙（1至1.5厘米），同侧桡骨保持完整。无一例出现间隙的自发桥接。间隙形成后6周，骨端变得光滑，并向同侧桡骨逐渐变细，观察到缺损被薄纤维组织填充。然后通过插入紧密贴合的同种异体骨基质植入物来桥接至少6周龄的陈旧性缺损。骨基质由健康兔的管状骨用0.6 M盐酸脱矿制备，保存在75%酒精中，并在3周内使用。通过四环素荧光在临床上、放射学上和组织学上观察植入物的转归长达12周。6周时大多数出现骨形成的放射学证据，植入后12周75%的病例可见间隙完全桥接。最初形成的大部分骨是编织骨，即直接由间充质细胞形成或在膜内形成的未成熟骨，不过在某些情况下也观察到软骨内成骨区域。到12周时，骨几乎已重塑为成熟管状骨的质地。75%的病例证实同种异体骨基质成功桥接慢性大块骨缺损。
