刘易斯大鼠对豚鼠和大鼠髓鞘碱性蛋白的肽C1（第68 - 88位氨基酸残基）的免疫反应。
Immune response of Lewis rats to peptide C1 (residues 68-88) of guinea pig and rat myelin basic proteins.
作者信息
Kibler R F, Fritz R B, Chou F, Peacocke N Y, Brown N M, McFarlin D E
出版信息
J Exp Med. 1977 Nov 1;146(5):1323-31. doi: 10.1084/jem.146.5.1323.
Peptide C1 (residues 68-88) from GP and rat BP differ by a single amino acid interchange at residue 79. This residue is serine in GP C1 and threonine in rat C1. GP C1 was encephalitogenic in Le rats at doses as low as 15 ng. Rat C1 was encephalitogenic at doses of 1,500 ng or greater. LNC from rats challenged with 25 X 10(-4) micronmol of GP C1 and 250 X 10(-4) micronmol of rat C1 showed a proliferative response in vitro to both peptides, but in each instance the magnitude of the response was greater to the GP peptide. GP C1 also induced higher levels of circulating antibodies at 25 X 10(-4) micronmol, but the specificity of antibodies produced by the two peptides was the same. These results have been interpreted as indicating that the presence of serine at position 79 in GP C1 results in the stimulation of greater numbers of T cells involved in (a) the induction of EAE, (b) the in vitro proliferative response and (c) helper function in antibody production.
来自糖蛋白（GP）的肽C1（第68 - 88位氨基酸残基）与大鼠碱性蛋白（BP）的肽C1在第79位氨基酸残基处存在单个氨基酸互换。该残基在GP C1中为丝氨酸，在大鼠C1中为苏氨酸。GP C1在Le大鼠中低至15纳克的剂量时就具有致脑炎性。大鼠C1在1500纳克或更高剂量时具有致脑炎性。用25×10⁻⁴微摩尔的GP C1和250×10⁻⁴微摩尔的大鼠C1攻击的大鼠的淋巴细胞（LNC）在体外对这两种肽均表现出增殖反应，但在每种情况下，对GP肽的反应程度更大。GP C1在25×10⁻⁴微摩尔时还诱导了更高水平的循环抗体，但两种肽产生的抗体特异性相同。这些结果被解释为表明GP C1中第79位的丝氨酸导致刺激了更多参与（a）实验性自身免疫性脑脊髓炎（EAE）诱导、（b）体外增殖反应和（c）抗体产生中的辅助功能的T细胞。
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