Geier M R, Stanbro H, Merril C R
Appl Environ Microbiol. 1978 Sep;36(3):445-9. doi: 10.1128/aem.36.3.445-449.1978.
Twenty samples of commercial vaccines intended for administration to humans were assayed for the presence of bacterial endotoxins by using the Limulus amebocyte lysate test. Sixteen of the vaccines contained more than 0.1 ng of endotoxin per ml (which corresponds to 103 bacterial cell wall equivalents per ml in the undiluted vaccines). These results suggest that at some stage of preparation, the vaccines have contained varying amounts of gram-negative bacteria and may indicate the presence of other bacterial products as well. It might be useful to list the level of endotoxins, phage, and other contaminants on each vaccine lot to facilitate studies on any side effects of these contaminants. Selection of vaccine lots with the least endotoxin might reduce some of the adverse effects of vaccinations.
采用鲎试剂法对20份拟用于人体的商用疫苗样本进行细菌内毒素检测。其中16份疫苗每毫升所含内毒素超过0.1纳克（相当于未稀释疫苗中每毫升有103个细菌细胞壁当量）。这些结果表明，在制备的某些阶段，疫苗含有不同数量的革兰氏阴性菌，也可能表明存在其他细菌产物。列出每批疫苗的内毒素、噬菌体和其他污染物水平，可能有助于研究这些污染物的任何副作用。选择内毒素含量最低的疫苗批次可能会减少一些疫苗接种的不良反应。
