Shibuya T, Cabrera B D, Tanaka H, Valeza F S, Instrella R
Jpn J Exp Med. 1980 Dec;50(6):463-8.
The blood film, Millipore filter and Nuclepore filter techniques were applied in a filariasis field survey at a village in the Philippines. venous blood from each person was examined by the three techniques to compare their efficacy of microfilariae detection. Overall positive rate was 16.1% by the combined techniques. Microfilaria positive rate was highest in Nuclepore filter method (15.6%) followed by Millipore technique (13.6%) and blood film (11.5%), but their difference was not significant statistically. The ratio of the total microfilaria counts by the three techniques was 1:29.8:30.3, which seems to reflect the ratio of the blood volumes used. There was no significant difference between the microfilaria counts by the three techniques adjusted to the same volume of 30 microliter and examined by F-test. It is considered that the blood film is still of practical value in field surveys.
在菲律宾的一个村庄进行丝虫病现场调查时应用了血涂片、微孔滤膜和核孔滤膜技术。对每个人的静脉血采用这三种技术进行检查，以比较它们检测微丝蚴的效果。联合技术的总体阳性率为16.1%。核孔滤膜法的微丝蚴阳性率最高（15.6%），其次是微孔滤膜技术（13.6%）和血涂片（11.5%），但它们之间的差异无统计学意义。三种技术检测到的微丝蚴总数之比为1:29.8:30.3，这似乎反映了所用血量的比例。将三种技术检测的微丝蚴数调整为相同体积30微升并通过F检验进行检查，结果无显著差异。认为血涂片在现场调查中仍具有实用价值。
