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[利用热解气相色谱法鉴别病毒结构蛋白]

[Differentiation of structural viral proteins using pyrolysis gas chromatography].

作者信息

Flunker G, Seidel W, Döhner L

出版信息

Arch Exp Veterinarmed. 1978;32(4):501-9.

PMID:727865
Abstract

Types 1, 2, and 5 of structural proteins of adenovirus as well as human albumin, pepsin, trypsin, and ribonuclease tend to exhibit characteristic substance patterns when exposed to pyrolysis-gas chromatography. Those patterns may be helpful in differentiating between those proteins. The presence of a distributional pattern is assumed to depend, in most cases, not only on the given amino acid composition, but also on the amino acid sequence of the protein proper. Samples of complex composition, such as complete viruses or allantois fluids, were much less suitable for differentiation by means of pyrolysis-gas chromatography.

摘要

腺病毒的1型、2型和5型结构蛋白以及人白蛋白、胃蛋白酶、胰蛋白酶和核糖核酸酶在进行热解气相色谱分析时往往会呈现出特征性的物质图谱。这些图谱可能有助于区分这些蛋白质。在大多数情况下,分布图谱的出现不仅取决于特定的氨基酸组成,还取决于蛋白质本身的氨基酸序列。成分复杂的样本,如完整病毒或尿囊液,不太适合通过热解气相色谱进行区分。

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