Iashina L N, Malygin E G
Mol Biol (Mosk). 1981 Jul-Aug;15(4):901-14.
Bifunctional DNA-polymerases catalysed matrix-dependent synthesis of DNA chains is accompanied by the correction of non-correct nucleotides incorporation through exonuclease excision. To study the models of the correction mechanism a method of stationary graphs was used. This method gives possibility to deduce easily the equations which describe the frequency of mistakes in the growing DNA chain as a function of DNA-polymerase activity. Two mechanisms of correction (mechanism of immediate correction and mechanism of following correction) are proved on the base of simple models of DNA chain biosynthesis on a homopolymer matrix with the participation of correct and non-correct substrates. Effect of kinetic parameters, characterizing the inclusion and elimination processes of correct and non-correct nucleotides, on the replication fidelity was analysed. One of two investigated models was chosen on the base of known experimental data.
双功能DNA聚合酶催化的依赖模板的DNA链合成过程伴随着通过核酸外切酶切除来校正错误掺入的核苷酸。为了研究校正机制的模型，使用了稳态图方法。该方法能够轻松推导描述正在生长的DNA链中错误频率作为DNA聚合酶活性函数的方程。基于在正确和错误底物参与下在均聚物模板上进行DNA链生物合成的简单模型，证明了两种校正机制（即时校正机制和后续校正机制）。分析了表征正确和错误核苷酸的掺入和去除过程的动力学参数对复制保真度的影响。根据已知实验数据选择了两个研究模型中的一个。
