多头绒泡菌细胞核中组蛋白H1与染色质的细胞周期依赖性解离。
Cell-cycle-dependent dissociation of histone H1 from chromatin in nuclei of P. polycephalum.
作者信息
Cruickshank H M, Walker I O
出版信息
Nucleic Acids Res. 1981 Aug 11;9(15):3873-85. doi: 10.1093/nar/9.15.3873.
The dissociation curves of histone H1 from chromatin in interphase and metaphase nuclei from Physarum polycephalum have been determined using CaCl2 as dissociating agent. H1 is less strongly bound to metaphase chromosomes than to interphase chromatin. However, no differences could be detected in the binding of Hl to early S, late S or G2 phase chromatin. The number of CaCl2 molecules involved in binding one H1 molecule to chromatin was reduced from 5 in interphase to 4 in metaphase. The non-electrostatic contribution to the free-energy of binding was small in both cases. A comparison of the binding properties of H1 to sheared chromatin, native chromatin and metaphase chromosomes suggests that the electrostatic binding functions of H1 are completely satisfied within the nucleosome and that further electrostatic interactions are not involved in folding the nucleosomal fibre into the 300 A "solenoid" or the more tightly folded metaphase chromosome.
以氯化钙作为解离剂，测定了多头绒泡菌间期核和中期核中染色质上组蛋白H1的解离曲线。与间期染色质相比，H1与中期染色体的结合较弱。然而，未检测到H1与早S期、晚S期或G2期染色质结合的差异。将一个H1分子与染色质结合所需的氯化钙分子数，从间期的5个减少到中期的4个。两种情况下，非静电作用对结合自由能的贡献都很小。对H1与剪切染色质、天然染色质和中期染色体结合特性的比较表明，H1的静电结合功能在核小体内已完全满足，将核小体纤维折叠成300埃的“螺线管”或更紧密折叠的中期染色体并不涉及进一步的静电相互作用。
Zotero 插件