Wilson S B
Biochem J. 1978 Oct 15;176(1):129-36. doi: 10.1042/bj1760129.
Freshly prepared washed or purified mung-bean (Phaseolus aureus) mitochondria utilize oxygen with ascorbate/tetramethyl-p-phenylenediamine mixture as electron donor in the presence of KCN. ATP control of the oxygen uptake can be observed with very fresh mitochondria. The electron flow, which is inhibited by antimycin A, salicylhydroxamic acid or octylguanidine, takes place by reversed electron transport through phosphorylation site II and thence to oxygen through the cyanide-insensitive pathway. Oligomycin and low concentrations of uncoupler partially inhibit the oxygen uptake in a manner similar to that observed for other energy-linked functions of plant mitochondria. An antimycin A-insensitive oxygen uptake occurs if high concentrations of uncoupler are used, indicating that the pathway of electron flow has been altered. The process of cyanide-insensitive ascorbate oxidation is self-starting, and, since it occurs in the presence of oligomycin, it is concluded that the reaction can be energized by a single energy-conservation site associated with the cyanide-insensitive oxidase pathway.
新鲜制备的洗涤或纯化绿豆（Phaseolus aureus）线粒体在KCN存在下，以抗坏血酸/四甲基对苯二胺混合物作为电子供体来利用氧气。使用非常新鲜的线粒体时，可以观察到氧气摄取的ATP控制。被抗霉素A、水杨羟肟酸或辛基胍抑制的电子流，通过磷酸化位点II的逆向电子传递发生，然后通过对氰化物不敏感的途径传递到氧气。寡霉素和低浓度的解偶联剂以类似于植物线粒体其他能量偶联功能所观察到的方式部分抑制氧气摄取。如果使用高浓度的解偶联剂，会出现抗霉素A不敏感的氧气摄取，这表明电子流途径已改变。氰化物不敏感的抗坏血酸氧化过程是自启动的，并且由于它在寡霉素存在下发生，所以得出结论，该反应可以由与氰化物不敏感氧化酶途径相关的单个能量保守位点提供能量。
