Pollak S, Vycudilik W
Wien Klin Wochenschr. 1981 Feb 20;93(4):111-7.
The pulmonary lipids were investigated at autopsy in 22 deaths from burns (3 scalds among them) by means of histological examination, thin-layer chromatography and gas liquid chromatography. Leaving out of consideration 4 cases with concomitant mechanical trauma 7 out of 18 burned victims showed fat embolism of the lungs, the degree varying from slight (4) to medium (3). Cases surviving for a short period showed fat embolism more frequently than those with a rapidly fatal course. The extracted pulmonary lipids were separated by thin-layer chromatography into 5 fractions (phospholipids, cholesterol, fatty acids, triglycerides, cholesterol esters) and evaluated densitometrically. The triglyceride fraction was increased in cases of fat embolism after burns, as well as in control cases of post-traumatic fat embolism. For further lipid characterisation the fatty acids were determined by gas chromatography. Calculation of the amounts of the individual fatty acids showed that in cases of at least medium intensity the ratio of oleic acid/linoleic acid and oleic acid/stearic acid is shifted towards oleic acid.
通过组织学检查、薄层色谱法和气液色谱法,对22例烧伤死亡病例(其中3例为烫伤)进行了尸检,以研究肺脂质情况。不考虑4例伴有机械性创伤的病例,18例烧伤患者中有7例出现了肺脂肪栓塞,程度从轻度(4例)到中度(3例)不等。短期存活的病例比那些迅速致命的病例更频繁地出现脂肪栓塞。提取的肺脂质通过薄层色谱法分离为5个组分(磷脂、胆固醇、脂肪酸、甘油三酯、胆固醇酯),并进行光密度测定评估。烧伤后脂肪栓塞病例以及创伤后脂肪栓塞对照病例中的甘油三酯组分均增加。为进一步进行脂质特征分析,通过气相色谱法测定脂肪酸。对各个脂肪酸含量的计算表明,在至少为中度强度的病例中,油酸/亚油酸和油酸/硬脂酸的比例向油酸偏移。