Newman M J, Brandon M R
Am J Vet Res. 1981 Jun;42(6):923-6.
Antibody responses following immunization with allogeneic bovine lymphocytes, goose red blood cells (GRBC), and ovalbumin (given IV) were studied in 20 heifers. Antibody to GRBC was detectable by 4 to 9 days in all heifers and followed the characteristic pattern of a low-titer primary response which was predominantly immunoglobulin (Ig) M, whereas a high titer IgG secondary response was observed. The dose of ovalbumin used failed to elicit a detectable antibody response. An immune response to allogeneic lymphocytes was observed, but it differed from the GRBC response. Cytotoxic antibody directed against lymphocytes was detectable by day 14 in 5 of the heifers. Five others responded by days 17 to 24 after secondary immunization. The responses were of low titer and predominantly IgM, after both primary and secondary immunizations. The time of antibody appearance and titers varied between heifers and was not dependent on differences at the major histocompatibility complex (BoLA) between heifers and immunizing lymphocytes. Cytotoxic antibody reacted with both B and T lymphocytes and appeared to recognize the serologically defined BoLA antigens. Noncytotoxic alloantibody was detected in the serum of 1 heifer and appeared to be directed against a subpopulation of lymphocytes, possibly those bearing Ia-like antigens.
对20头小母牛进行了研究，观察其在用同种异体牛淋巴细胞、鹅红细胞（GRBC）和卵清蛋白（静脉注射）免疫后的抗体反应。所有小母牛在4至9天内均可检测到针对GRBC的抗体，且呈现出低滴度主要为免疫球蛋白（Ig）M的初次反应特征模式，而观察到的是高滴度IgG的二次反应。所用卵清蛋白剂量未能引发可检测到的抗体反应。观察到对同种异体淋巴细胞的免疫反应，但与对GRBC的反应不同。在14天时，5头小母牛可检测到针对淋巴细胞的细胞毒性抗体。另外5头在二次免疫后17至24天出现反应。初次和二次免疫后，反应滴度均较低且主要为IgM。抗体出现的时间和滴度在不同小母牛之间有所差异，且不取决于小母牛与免疫淋巴细胞之间主要组织相容性复合体（BoLA）的差异。细胞毒性抗体与B淋巴细胞和T淋巴细胞均发生反应，似乎识别血清学定义的BoLA抗原。在1头小母牛的血清中检测到非细胞毒性同种异体抗体，似乎针对淋巴细胞亚群，可能是那些携带Ia样抗原的淋巴细胞。
