Shellito J, Murphy S, Warner N
Am Rev Respir Dis. 1981 Sep;124(3):333-6. doi: 10.1164/arrd.1981.124.3.333.
Lavaged lung cells from normal rabbits and rabbits given hydrochloric acid intratracheally were analyzed using flow cytometry techniques. The rabbit alveolar macrophage was characterized as a cell with relatively high light scatter and high intrinsic autofluorescence compared with the lung neutrophil with its lower light scatter and autofluorescence. The flow cytometry characteristics of these 2 cell populations were confirmed with light microscopy after cell sorting. A flow cytometry analysis of alveolar macrophage phagocytosis using fluorescent latex particles was applied to normal rabbit alveolar macrophages. These studies showed that flow cytometry can be used to study the morphologic features and function of rabbit lavage lung cell populations and may be ultimately useful in the analysis of human lung cells.
使用流式细胞术技术分析了正常兔和经气管内给予盐酸的兔的灌洗肺细胞。与肺中性粒细胞较低的光散射和自发荧光相比，兔肺泡巨噬细胞的特征是具有相对较高的光散射和较高的固有自发荧光。细胞分选后通过光学显微镜确认了这两种细胞群的流式细胞术特征。将使用荧光乳胶颗粒对肺泡巨噬细胞吞噬作用进行的流式细胞术分析应用于正常兔肺泡巨噬细胞。这些研究表明，流式细胞术可用于研究兔灌洗肺细胞群的形态学特征和功能，最终可能有助于分析人肺细胞。
