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[无神经胶质的分离神经元培养物中活神经末梢的反应性变化]

[Reactive changes in living nerve endings in cultures of isolated neurons devoid of glia].

作者信息

Sotnikov O S, Kostenko M A

出版信息

Arkh Anat Gistol Embriol. 1981 Jun;80(6):17-26.

PMID:7283764
Abstract

Reactive changes in terminal apparatus of mollusc mature neurons treated with pronase and cultivated in 20% medium with saline solutions added have been studied by means of modern methods of differential and coloured interferential contrast. The culture used can serve to study mechanisms of an urgent nonspecific reaction of varicose rearrangement of the terminal apparatus, as well as delayed reactive changes of an "excessive" growth type, autapses formation, self amputation etc. In the glia-deprived culture, glia is not an initial organizing factor of varicose deformity of the processes. Mechanisms of varicose rearrangement of thick and thin fibres are discussed.

摘要

利用现代微分和彩色干涉对比方法,研究了用链霉蛋白酶处理并在添加盐溶液的20%培养基中培养的软体动物成熟神经元终末装置的反应性变化。所使用的培养物可用于研究终末装置曲张重排的紧急非特异性反应机制,以及“过度”生长类型的延迟反应性变化、自突触形成、自我切断等。在无神经胶质细胞的培养物中,神经胶质细胞不是突起曲张畸形的初始组织因子。文中讨论了粗细纤维曲张重排的机制。

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