Macdougall A J, Brown J R, Plumbridge T W
Biochem J. 1980 Dec 1;191(3):855-8. doi: 10.1042/bj1910855.
A method is described in which double-stranded DNA is alkylated with 4-bis-(2-chloroethyl)amino-L-phenylalanine and the product immobilized on an insoluble support via the primary amino group of the phenylalanine moiety. The DNA is hence irreversibly bound to the matrix by both strands at a limited number of points.
描述了一种方法,其中双链DNA用4-双(2-氯乙基)氨基-L-苯丙氨酸进行烷基化,产物通过苯丙氨酸部分的伯氨基固定在不溶性载体上。因此,DNA在有限数量的位点上通过两条链不可逆地结合到基质上。