由通过双环氧乙烷活化多糖固定的核酸组成的亲和吸附剂。
Affinity adsorbents consisting of nucleic acids immobilized via bisoxirane activated polysaccharides.
作者信息
Potuzak H, Dean P D
出版信息
Nucleic Acids Res. 1978 Jan;5(1):297-303. doi: 10.1093/nar/5.1.297.
Abstract
An easy and efficient procedure for the immobilization of polynucleotide ligands to bisoxirane activated insoluble polysaccharides has been elaborated and is described in this paper. The resulting materials have been applied to the chromatography of DNA polymerase I, and RNA polymerase from E.coli. Because of their extraordinary stability to temperature, formamide, and alkaline conditions they seem to be particularly useful adsorbents for nucleic acid hybridization.
摘要
本文详细阐述并描述了一种将多核苷酸配体固定到双环氧乙烷活化的不溶性多糖上的简便高效方法。所得材料已应用于大肠杆菌DNA聚合酶I和RNA聚合酶的色谱分析。由于它们对温度、甲酰胺和碱性条件具有非凡的稳定性，它们似乎是核酸杂交特别有用的吸附剂。
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