Stumph W E, Wu J R, Bonner J
Biochemistry. 1978 Dec 26;17(26):5791-8. doi: 10.1021/bi00619a028.
Antibodies, shown to be specific for DNA/RNA hybrids, have been covalently attached to CNBr-activated Sepharose. The resultant affinity resin specifically binds DNA/RNA hybrids and has been used to enrich for the DNA which codes for rRNA in the slime mold Dictyostelium discoideum. By utilizing the technique of R-loop formation, DNA molecules containing the rRNA genes were isolated from total nuclear DNA in a double-stranded form. These rDNA molecules, which were recovered by high salt elution from the affinity resin, were typically 15-40 kbp in length, and thus contained DNA sequences adjacent to the selected sequences coding for the 17S and 26S rRNAs. In addition, evidence has been obtained concerning the structure of Dictyostelium rDNA which agrees with the finding (Taylor et al. (1977) ICN-UCLA Symp. Mol. Cell. Biol. 8, 309-313) that the rDNA molecules are not covalently attached to the chromosomes of this organism.
已证明对DNA/RNA杂交体具有特异性的抗体被共价连接到溴化氰活化的琼脂糖凝胶上。所得的亲和树脂能特异性结合DNA/RNA杂交体，并已用于富集编码黏菌盘基网柄菌核糖体RNA的DNA。通过利用R环形成技术，以双链形式从总核DNA中分离出含有核糖体RNA基因的DNA分子。这些通过高盐洗脱从亲和树脂上回收的核糖体DNA分子长度通常为15 - 40千碱基对，因此包含与编码17S和26S核糖体RNA的选定序列相邻的DNA序列。此外，已获得有关盘基网柄菌核糖体DNA结构的证据，这与核糖体DNA分子不与该生物体的染色体共价连接这一发现（泰勒等人（1977年）《国际癌症研究机构 - 加州大学洛杉矶分校分子与细胞生物学研讨会》第8卷，第309 - 313页）一致。
