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核酸与多胺之间的相互作用。I. 亚精胺与5'-腺苷酸的高分辨率碳-13和氢-1核磁共振研究。

The interactions between nucleic acids and polyamines. I. High resolution carbon-13 and hydrogen-1 nuclear magnetic resonance studies of spermidine and 5'-AMP.

作者信息

Bunce S, Kong E S

出版信息

Biophys Chem. 1978 Sep;8(4):357-68. doi: 10.1016/0301-4622(78)80017-9.

DOI:10.1016/0301-4622(78)80017-9
PMID:728538
Abstract

In 0.5 M solution at pH 7.6, interaction of spermidine and 5'-AMP is demonstrated by downfield proton NMR shifts. Shifts of ribose and adenine protons support a model in which triprotonated spermidine engages the phosphate anion with the C-3 diamine segment in a conformation to maximize interaction and the C-4 amino segment extended to interact with adenine N-7 (base anti, 2' endo, g'g' and gg nucleoside conformation). Changes in carbon-13 chemical shifts fro ribose C-5' (downfield), C-2' C-3', and C-4' (upfield) and for adenine C-6 and C-8 (upfield) support this model. In 0.006 M solution no significant changes in proton shifts and therefore no evidence for interaction was found. Spermidine and 5' -UMP (0.006 M) showed interaction at pH 10.5 (small upfield shifts in the proton nmr) interpreted as changing conformation by solvent interaction. In 0.00l M 3'-UMP at pH 10.5 small downfield proton shifts induced by spermidine are attributed to interactions with phosphate anion.

摘要

在pH值为7.6的0.5 M溶液中，通过质子核磁共振谱的低场位移证明了亚精胺与5'-AMP之间的相互作用。核糖和腺嘌呤质子的位移支持了这样一种模型：三质子化的亚精胺通过C-3二胺片段与磷酸根阴离子结合，其构象可使相互作用最大化，而C-4氨基片段则伸展以与腺嘌呤的N-7相互作用（碱基反式，2'内式，g'g'和gg核苷构象）。核糖C-5'（低场）、C-2'、C-3'和C-4'（高场）以及腺嘌呤C-6和C-8（高场）的碳-13化学位移变化支持了该模型。在0.006 M溶液中，质子位移没有显著变化，因此未发现相互作用的证据。亚精胺与5'-UMP（0.006 M）在pH 10.5时显示出相互作用（质子核磁共振谱中有小的高场位移），这被解释为通过溶剂相互作用改变了构象。在pH 10.5的0.001 M 3'-UMP中，亚精胺引起的小的低场质子位移归因于与磷酸根阴离子的相互作用。

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