Gupta K G, Yadav N K, Dhawan S
Biotechnol Bioeng. 1978 Dec;20(12):1895-901. doi: 10.1002/bit.260201206.
Conditions for the laboratory-scale production of acetoin plus diacetyl by Enterobacter cloacae ATCC 27613 were studied. Thirty-five g acetoin plus diacetyl/50 g sucrose were obtained when fermentation was carried out in 2.5 liter medium containing 12.5 g peptone and 12.5 g yeast extract, at pH 7.0, in a 5 liter conical flask on a shaker (240 rpm) at 28-30 degrees C for 48 hr. Recovery of pure diacetyl was 85% of the total acetoin plus diacetyl.
研究了阴沟肠杆菌ATCC 27613实验室规模生产乙偶姻加双乙酰的条件。在含有12.5 g蛋白胨和12.5 g酵母提取物的2.5升培养基中，于pH 7.0、5升锥形瓶中、摇床（240转/分钟）、28 - 30℃下进行48小时发酵，可获得35 g乙偶姻加双乙酰/50 g蔗糖。纯双乙酰的回收率为乙偶姻加双乙酰总量的85%。
