Roos K P, Baskin R J
Comput Programs Biomed. 1981 Mar-Jun;13(1-2):33-42. doi: 10.1016/0010-468x(81)90082-9.
Transient diffraction pattern parameter and tension responses were recorded from isolated frog semitendinosus muscle fibers subjected to quick stretches and release with an on-line digital acquisition system. The sarcomere length was determined from the zero to first order diffraction line spacing detected with a charge-coupled device photosensing array. Diffraction data was digitized and sequentially stored every 18 ms by a PDP-8 machine language program for later analysis. Sarcomere length, diffraction line intensity, and sarcomere length dispersion were determined with an 8080A microcomputer program incorporating extended math and floating point software packages. Tension records were simultaneously photographed from a storage oscilloscope. Quick stretches and release on tetanically stimulated single skeletal muscle fibers indicated that the transient sarcomere length change occurred in less than 18 ms. Transient tension responses demonstrated a long (150-200 ms) tension recovery phase with no corresponding sarcomere length change. There were no significant alterations in the diffraction line intensity or dispersion following the length perturbations.
利用在线数字采集系统，记录了经快速拉伸和释放处理的离体青蛙半腱肌纤维的瞬态衍射图谱参数和张力响应。肌节长度由电荷耦合器件光敏阵列检测到的零级到一级衍射线间距确定。衍射数据经数字化处理后，由一个PDP - 8机器语言程序每18毫秒顺序存储一次，以备后续分析。肌节长度、衍射线强度和肌节长度离散度通过一个包含扩展数学和浮点软件包的8080A微机程序来确定。张力记录同时从存储示波器上拍摄下来。对强直刺激的单根骨骼肌纤维进行快速拉伸和释放表明，瞬态肌节长度变化发生在不到18毫秒的时间内。瞬态张力响应显示出一个较长（150 - 200毫秒）的张力恢复阶段，而肌节长度没有相应变化。长度扰动后，衍射线强度或离散度没有显著改变。
