Biscoglio de Jimenez Bonino M J, Cascone O, Arnao de Nuĕ A I, Santomé J A, Sánchez D, Oré R, Villavicencio M
Int J Pept Protein Res. 1981 Mar;17(3):374-9. doi: 10.1111/j.1399-3011.1981.tb02004.x.
A simple and mild procedure is described for the isolation of pituitary growth hormone from alpaca glands. It involves extractions in buffer at pH 8.7 and gel filtration in Sephadex G-75. an average yield of 1 mg purified product may be obtained from 1 g fresh alpaca pituitary glands. The alpaca hormone was examined for homogeneity by SDS-polyacrylamide gel electrophoresis, gel electro-focusing and end-group analyses. It is a protein composed of a single polypeptide chain with phenylalanine at both ends. The C-terminal sequence is -Ala-Phe. The molecular weight is approximately 21 700 and the amino acid composition very similar to that observed in equine growth hormone.
本文描述了一种从羊驼腺体中分离垂体生长激素的简单温和方法。该方法包括在pH 8.7的缓冲液中提取以及在葡聚糖凝胶G-75上进行凝胶过滤。从1克新鲜羊驼垂体腺体中平均可获得1毫克纯化产物。通过SDS-聚丙烯酰胺凝胶电泳、凝胶电聚焦和端基分析对羊驼激素的纯度进行了检测。它是一种由单条多肽链组成的蛋白质，两端均为苯丙氨酸。C端序列为-Ala-Phe。分子量约为21700，氨基酸组成与马生长激素非常相似。
