Winterlin W, Hall G, Mourer C
J Assoc Off Anal Chem. 1981 Sep;64(5):1055-9.
A simple and sensitive procedure is presented for the determination of furazolidone in turkey tissues, using liquid partitioning followed by high performance liquid chromatography (HPLC). Fat, liver, kidney, skin, and muscle tissues are ground with methylene chloride in a Polytron homogenizer, followed by solvent removal, partitioning in hexane-0.01M acetic acid, and back-partitioning the 0.01M acetic acid with methylene chloride. The determination by HPLC used a reverse phase Ultrasphere-ODS 5 micrometer column. The method is sensitive to 0.5 ppb, with a standard deviation of 6.39% at the 2 ppb fortification level. Recovery from fortified tissues averaged 84% from samples fortified with 0.5-10 ppb furazolidone. An alternative cleanup procedure using a Sep-Pak C18 cartridge is also presented.
本文介绍了一种简单且灵敏的方法，用于测定火鸡组织中的呋喃唑酮，该方法采用液液分配后进行高效液相色谱（HPLC）分析。将脂肪、肝脏、肾脏、皮肤和肌肉组织在Polytron匀浆器中与二氯甲烷研磨，随后去除溶剂，在己烷 - 0.01M乙酸中进行分配，并用二氯甲烷对0.01M乙酸进行反萃取。HPLC测定使用反相Ultrasphere - ODS 5微米柱。该方法对0.5 ppb敏感，在2 ppb强化水平下的标准偏差为6.39%。用0.5 - 10 ppb呋喃唑酮强化的组织样品平均回收率为84%。本文还介绍了一种使用Sep - Pak C18柱的替代净化方法。
