Rosenberg M, Rosenberg E
J Bacteriol. 1981 Oct;148(1):51-7. doi: 10.1128/jb.148.1.51-57.1981.
The high affinity of Acinetobacter calcoaceticus RAG-1 for liquid hydrocarbons permitted the isolation of a spontaneous nonadherent mutant, MR-481. Strain MR-481 exhibited no significant affinity for three test hydrocarbons, yet resembled the wild type in many properties, including production of the extracellular emulsifying agent emulsan. To study the role of adherence in growth on hydrocarbons, RAG-1 and MR-481 were compared for growth on hexadecane under conditions of limited agitation and at low initial cell densities. Adherent RAG-1 cells were able to grow rapidly under these conditions, whereas nonadherent MR-481 cells failed to grow for at least 54 h. However, the addition of emulsan either initially or at various times after inoculation enabled the nonadherent MR-481 cells to grow on hexadecane. Growth was not the result of reversion of MR-481 from nonadherent to adherent cells. The data demonstrate that adherence is a crucial factor in the growth of A. calcoaceticus RAG-1 on hexadecane in the absence of extracellular emulsification of the substrate.
醋酸钙不动杆菌RAG-1对液态烃具有高亲和力，这使得一种自发的非粘附突变体MR-481得以分离。MR-481菌株对三种测试烃没有明显的亲和力，但在许多特性上与野生型相似，包括细胞外乳化剂乳化素的产生。为了研究粘附在烃类生长中的作用，在搅拌受限和低初始细胞密度条件下，比较了RAG-1和MR-481在十六烷上的生长情况。粘附性的RAG-1细胞在这些条件下能够快速生长，而非粘附性的MR-481细胞至少54小时未能生长。然而，在接种后最初或不同时间添加乳化素能使非粘附性的MR-481细胞在十六烷上生长。生长并非MR-481从非粘附细胞转变为粘附细胞的结果。数据表明，在底物没有细胞外乳化的情况下，粘附是醋酸钙不动杆菌RAG-1在十六烷上生长的关键因素。
