Omura S, Minami S, Tanaka H
J Biochem. 1981 Jul;90(1):291-3. doi: 10.1093/oxfordjournals.jbchem.a133465.
The enzyme reactions from NNM-A to NNM-E and from NNM-E to NNM-B were established in a cell-free system containing an extract from the nanaomycin producer Streptomyces rosa var. notoensis. The enzyme which catalyses the former epoxide-forming reaction (NNM-A leads to NNM-E) required NADH (or NADPH) and O2 suggesting that it is the monooxygenase-type; thus, NNM-A monooxygenase (4a,10a-epoxidizing) is proposed as its name. The other enzyme which catalyses the reductive epoxide-opening reaction (NNM-E leads to NNM-B) requires NADH or NADPH and is tentatively named NNM-B synthetase. Such a reductive epoxide-opening reaction is a novel enzyme reaction.
在含有纳纳霉素产生菌诺托玫瑰链霉菌提取物的无细胞体系中，确立了从NNM-A到NNM-E以及从NNM-E到NNM-B的酶促反应。催化前一个环氧化反应（NNM-A生成NNM-E）的酶需要NADH（或NADPH）和O₂，这表明它是单加氧酶类型；因此，将其命名为NNM-A单加氧酶（4a,10a-环氧化）。催化还原开环反应（NNM-E生成NNM-B）的另一种酶需要NADH或NADPH，暂命名为NNM-B合成酶。这种还原开环反应是一种新型酶促反应。
