Jabs C M, Ferrell W J, Robb H J
Clin Biochem. 1978 Oct;11(5):190-3. doi: 10.1016/s0009-9120(78)80027-7.
A method is described for the determination of low plasma levels of adenosine-5'-diphosphate (ADP) using a Dupont Biometer to measure luminescence produced by the luciferin-luciferase reaction. Endogenous ATP is removed by incubation with luciferase. The remaining ADP is then quantitated, following its conversion to ATP, after incubation with creatine phosphate and creatine kinase. The mean coefficient of variation for 0.02 and 2.2 micromol/liter ADP standards were 2.1 and 1.8% respectively. The method has been applied to human and rabbit plasma. Human plasma ADP concentrations were found to be 0.13 +/- 0.025 (10) micromol/liter and rabbit plasma concentration were 0.07 +/- 0.05 (5) micromol/liter. Several other possible applications of the method are discussed.
描述了一种使用杜邦生物计测定血浆中低水平5'-二磷酸腺苷（ADP）的方法，该方法通过测量荧光素-荧光素酶反应产生的发光来进行。通过与荧光素酶孵育去除内源性ATP。然后，在与磷酸肌酸和肌酸激酶孵育后，将剩余的ADP转化为ATP后进行定量。0.02和2.2微摩尔/升ADP标准品的平均变异系数分别为2.1%和1.8%。该方法已应用于人和兔血浆。发现人血浆ADP浓度为0.13±0.025（10）微摩尔/升，兔血浆浓度为0.07±0.05（5）微摩尔/升。还讨论了该方法的其他几种可能应用。
