Gallin E K
Science. 1981 Oct 23;214(4519):458-60. doi: 10.1126/science.7291986.
Voltage clamp studies of macrophages from cultures of mouse spleen macrophages produced N-shaped steady-state current-voltage curves containing a region of negative slope resistance. Some macrophages exhibit two stable states of membrane potential, having current-voltage relationships that cross the voltage axis at three points. Outward currents that turn on at voltages of +15 millivolts or greater were noted in several cells. The addition of barium chloride to the bathing medium abolished the negative slope resistance and reduced the inward currents in response to hyperpolarizing voltage steps. These data provide direct evidence that macrophages exhibit at least tow different voltage-dependent conductances and demonstrate that voltage clamp techniques can be useful in studying the membrane properties of leukocytes.
对从小鼠脾脏巨噬细胞培养物中获取的巨噬细胞进行电压钳研究，得到了呈N形的稳态电流-电压曲线，其中包含一个负斜率电阻区域。一些巨噬细胞表现出两种稳定的膜电位状态，其电流-电压关系在三个点与电压轴相交。在几个细胞中记录到在+15毫伏或更高电压时开启的外向电流。向浴液中添加氯化钡消除了负斜率电阻，并减少了对超极化电压阶跃的内向电流。这些数据提供了直接证据，表明巨噬细胞至少表现出两种不同的电压依赖性电导，并证明电压钳技术可用于研究白细胞的膜特性。
