大鼠尿液、粪便和胆汁中区域异构体单羟基二苯甲烷代谢物的半定量分析。

Semi-quantitative profile of regioisomeric monohydroxydiphenylmethane metabolites in rat urine, faeces and bile.

Stocklinski A W

Xenobiotica. 1981 Jun;11(6):425-32. doi: 10.3109/00498258109045852.

Abstract

Urine and faeces, and two-hour bile samples from adult male rats dosed with [14C]diphenylmethane were analysed for benzhydrol and 2- and 4-hydroxydiphenyl-methane by silica gel GF t.l.c. and 14C-determination. 2. Mean values of 48.4% and 17.7% of the administered 14C were present in 24 h urine and faeces, respectively. Benzhydrol and 2- and 4-hydroxydiphenylmethane comprised 3.7%, 0.3% and 4.8% respectively of the 14C isolated from urine and 3.1%, 0.8%, and 4.8% respectively of the 14C isolated from faeces. 3. Bile samples (2h) contained 3.2% of the administered radioactivity. After treatment with beta-glucuronidase/aryl sulphatase, 71.9% of the recovered biliary radioactivity was identified as benzhydrol (37.5%) and 4-hydroxydiphenylmethane (34.4%).

摘要

对用[14C]二苯甲烷给药的成年雄性大鼠的尿液、粪便以及两小时胆汁样本，通过硅胶GF薄层层析法和14C测定法分析二苯甲醇、2-羟基二苯甲烷和4-羟基二苯甲烷。2. 给药的14C分别有48.4%和17.7%的平均值存在于24小时尿液和粪便中。从尿液中分离出的14C中二苯甲醇、2-羟基二苯甲烷和4-羟基二苯甲烷分别占3.7%、0.3%和4.8%，从粪便中分离出的14C中它们分别占3.1%、0.8%和4.8%。3. 胆汁样本（2小时）含有给药放射性的3.2%。用β-葡萄糖醛酸酶/芳基硫酸酯酶处理后，回收的胆汁放射性中71.9%被鉴定为二苯甲醇（37.5%）和4-羟基二苯甲烷（34.4%）。