Karjalainen H E, Mäntyjärvi R A
Acta Pathol Microbiol Scand C. 1981 Feb;89(1):49-54. doi: 10.1111/j.1699-0463.1981.tb02664.x.
A complement-mediated microcytotoxicity assay was used to detect Forssman antigen in BK virus (BKV)-induced tumor cells. BKT-1B, a transplantable hamster tumor cell line, contained Forssman antigen as did some other hamster cell lines tested for comparison. A rat embryo cell line transformed by BKV in vitro was negative for Forssman antigen. The presence of forssman antigen in hamster cell lines was confirmed by absorption experiments of rabbit forssman antiserum with the tumor cells, although in quantitative terms the results obtained by the two techniques were somewhat different. Immunization of rabbits with tumor cells also induced production of Forssman antibody; a much weaker response was observed in hamsters and rats.
采用补体介导的微量细胞毒性试验检测BK病毒（BKV）诱导的肿瘤细胞中的福斯曼抗原。可移植的仓鼠肿瘤细胞系BKT-1B含有福斯曼抗原，其他一些用于比较测试的仓鼠细胞系也含有该抗原。体外被BKV转化的大鼠胚胎细胞系对福斯曼抗原呈阴性。通过兔福斯曼抗血清与肿瘤细胞的吸收实验证实了仓鼠细胞系中存在福斯曼抗原，不过从定量角度来看，两种技术获得的结果略有不同。用肿瘤细胞免疫兔子也可诱导产生福斯曼抗体；在仓鼠和大鼠中观察到的反应则弱得多。
