Lavin M F, Good M, Counsilman C, Kidson C
Aust J Exp Biol Med Sci. 1981 Aug;59(4):515-20. doi: 10.1038/icb.1981.44.
A human melanoma cell line (MM253) was found to be sensitive to ultraviolet (UV) radiation, having a Do of 1.0 J/M2 when cloned on plastic culture dishes and a Do of 2.3 J/m2 when cloned in agar. These figures are much lower than those obtained for all other human melanoma cell lines studied in this laboratory (Do of 32-40 J/m2) and demonstrate that MM253 is unusually UV sensitive. The increased level of UV sensitivity in MM253 is not due to a reduced capacity for excision of pyrimidine dimers, repair of DNA single-strand breaks or elongation of newly-synthesized DNA strands when comparison is made with a UV-resistant melanoma cell line.
一种人类黑色素瘤细胞系（MM253）被发现对紫外线（UV）辐射敏感，当接种于塑料培养皿上时，其D0值为1.0 J/M2，而接种于琼脂中时，D0值为2.3 J/m2。这些数值远低于本实验室研究的所有其他人类黑色素瘤细胞系所获得的数值（D0值为32 - 40 J/m2），表明MM253对紫外线异常敏感。与一种抗紫外线的黑色素瘤细胞系相比，MM253中紫外线敏感性的增加并非由于嘧啶二聚体切除能力降低、DNA单链断裂修复能力降低或新合成DNA链延伸能力降低所致。
