Bougis P, Rochat H, Piéroni G, Verger R
Biochemistry. 1981 Aug 18;20(17):4915-20. doi: 10.1021/bi00520a017.
The monomolecular film technique was used to compare the specific interaction of four cardiotoxins from Naja mossambica mossambica with different phospholipids. We were able to demonstrate the interaction of cardiotoxins (10(-7) M) with both neutral and negatively charged phospholipids up to very high surface pressures (45 dyn/cm). In the presence of a phospholipid monolayer, the surface activity of cardiotoxins became much greater than that observed at the air-water interface. Neurotoxins of the same venom do not penetrate a phospholipid film, even at low surface pressure (15 dyn/cm). The apparent molecular area of cardiotoxin III during its insertion into a negatively charged phospholipid film was quantitatively defined. As a function of surface pressure of the membrane around 25 dyn/cm, cardiotoxins may exist in two different configurations, "flat" (1400 A2) or "edgewise" (420 A2). This result could account for the lytic activity of this type of toxin.
采用单分子膜技术比较了来自莫桑比克射毒眼镜蛇的四种心脏毒素与不同磷脂的特异性相互作用。我们能够证明心脏毒素（10⁻⁷ M）与中性和带负电荷的磷脂之间的相互作用，直至非常高的表面压力（45达因/厘米）。在磷脂单层存在的情况下，心脏毒素的表面活性比在空气-水界面观察到的要大得多。同一毒液中的神经毒素即使在低表面压力（15达因/厘米）下也不会穿透磷脂膜。定量确定了心脏毒素III插入带负电荷的磷脂膜过程中的表观分子面积。作为膜表面压力在25达因/厘米左右的函数，心脏毒素可能以两种不同的构型存在，“平躺”（1400 Ų）或“边缘”（420 Ų）。这一结果可以解释这类毒素的裂解活性。
