Bhavanandan V P
Biochemistry. 1981 Sep 15;20(19):5595-602. doi: 10.1021/bi00522a037.
The glycosaminoglycans produced by human fetal uveal melanocytes and by human melanoma cells were examined. The cells were grown in the presence of [3H]glucosamine and [35S]sulfate, and the labeled glycosaminoglycans were isolated from the cells, spend medium, and intracellular material. The distribution of the glycosaminoglycans was similar in both cells and spent media, which together accounted for 95% of the total. Of the total 3H]labeled glycosaminoglycans produced by the melanocyte culture, 42% was in chondroitin 4-sulfate, 25% in heparan sulfate, 16% in chondroitin 6-sulfate, and 17% in hyaluronic acid. In contrast, HM7 human melanoma cultures produced no chondroitin 6-sulfate, increased quantities of heparan sulfate, and less hyaluronic acid. A heparan sulfate fraction obtained from melanocytes required both heparitinase and heparinase for complete degradation, indicating the presence of heparin-like molecules in this fraction. The corresponding fraction from melanoma cells was totally degraded by heparitinase alone.
对人胎儿葡萄膜黑色素细胞和人黑色素瘤细胞产生的糖胺聚糖进行了检测。细胞在含有[³H]葡糖胺和[³⁵S]硫酸盐的条件下生长，然后从细胞、消耗培养基和细胞内物质中分离出标记的糖胺聚糖。糖胺聚糖在细胞和消耗培养基中的分布相似，二者加起来占总量的95%。黑色素细胞培养物产生的总[³H]标记糖胺聚糖中，42%为硫酸软骨素4-硫酸盐，25%为硫酸乙酰肝素，16%为硫酸软骨素6-硫酸盐，17%为透明质酸。相比之下，HM7人黑色素瘤培养物不产生硫酸软骨素6-硫酸盐，硫酸乙酰肝素的量增加，透明质酸减少。从黑色素细胞获得的硫酸乙酰肝素组分需要同时用乙酰肝素酶和肝素酶才能完全降解，表明该组分中存在类肝素分子。黑色素瘤细胞的相应组分仅用乙酰肝素酶就能完全降解。
