分泌抗角膜抗体的杂交瘤的制备。

Production of hybridomas secreting antibodies to the cornea.

作者信息

Zam Z S, Jones P, Das N D

出版信息

Curr Eye Res. 1981;1(3):139-44. doi: 10.3109/02713688109001819.

Abstract

Major controversies exist in the literature on the presence of blood group antigens on the endothelial and stromal layers of the cornea, and the importance of major histocompatibility typing for keratoplasty. Antibodies were raised in BALB/C mice against water soluble proteins of corneal epithelium. Following fusion of spleen cells with myeloma cells (Sp2/0-Ag14) hybrid colonies were maintained in HAT selective medium. The supernates of each colony were measured and screened for antibody production by radioimmunoassay. Gel electrophoresis of the antigen showed nine major bands. The antibodies were partially characterized by cross reaction against other soluble corneal fractions.

摘要

关于角膜内皮和基质层上血型抗原的存在以及主要组织相容性分型在角膜移植中的重要性，文献中存在重大争议。在BALB/C小鼠中产生了针对角膜上皮水溶性蛋白的抗体。脾细胞与骨髓瘤细胞（Sp2/0-Ag14）融合后，杂交菌落保存在HAT选择培养基中。通过放射免疫测定法测量每个菌落的上清液并筛选抗体产生。抗原的凝胶电泳显示出九条主要条带。通过与其他可溶性角膜组分的交叉反应对抗体进行了部分表征。