Zam Z S, Das N D, Jones P, Stern G A
Curr Eye Res. 1982;2(3):187-90. doi: 10.3109/02713688208997693.
Human corneal epithelial water insoluble proteins were used to immunize mice. Immune splenocytes were fused with Sp 2/0-Ag14 mouse myeloma cells by 40% PEG. Hybridoma antibodies obtained by somatic cell fusion were tested by radioimmunoassay. Supernatants from antibody positive hybrid colonies were used in immunofluorescence and crossreaction assays to locate and characterize corneal epithelial antigens. At least three distinct epithelial cell antigens were detected, one of which cross-reacts with rabbit corneal epithelial cells.
用人角膜上皮水不溶性蛋白免疫小鼠。免疫脾细胞与Sp 2/0-Ag14小鼠骨髓瘤细胞通过40%聚乙二醇进行融合。通过体细胞融合获得的杂交瘤抗体用放射免疫测定法进行检测。抗体阳性杂交集落的上清液用于免疫荧光和交叉反应测定，以定位和鉴定角膜上皮抗原。至少检测到三种不同的上皮细胞抗原，其中一种与兔角膜上皮细胞发生交叉反应。
