Bland P W, Britton D C, Richens E R, Pledger J V
Gut. 1981 Sep;22(9):744-51. doi: 10.1136/gut.22.9.744.
A reliable technique has been devised for the preparation of colorectal tumour-infiltrating lymphocytes (TIL). The immune capacity of these lymphocytes has been assessed in vitro and compared with that of lymphocytes infiltrating the lamina propria of adjacent normal mucosa (LPL) and with autologous peripheral blood lymphocytes (PBL). Assay of a natural killer (NK) cell function revealed the absence of such activity in TIL and LPL depsite the presence of normal levels in PBL. Antibody-dependent (K cell) cytotoxic activity was also absent in TIL and LPL. Both TIL and LPL showed significant mitogen-induced cytotoxic responses, although higher levels were detected in PBL. Tumour-infiltrating lymphocytes revealed depressed levels of spontaneous DNA synthesis, but mitogen stimulation of TIL was equivalent to that of LPL. T-cell proportions in TIL preparations were equivalent to those in PBL, but LPL comprised significantly fewer T cells.
已设计出一种可靠的技术用于制备结直肠肿瘤浸润淋巴细胞(TIL)。已在体外评估了这些淋巴细胞的免疫能力,并与浸润相邻正常黏膜固有层的淋巴细胞(LPL)以及自体外周血淋巴细胞(PBL)的免疫能力进行了比较。自然杀伤(NK)细胞功能检测显示,尽管PBL中NK细胞活性水平正常,但TIL和LPL中均不存在此类活性。TIL和LPL中也不存在抗体依赖性(K细胞)细胞毒性活性。TIL和LPL均显示出显著的丝裂原诱导的细胞毒性反应,尽管在PBL中检测到的水平更高。肿瘤浸润淋巴细胞显示出自发性DNA合成水平降低,但TIL的丝裂原刺激与LPL相当。TIL制剂中的T细胞比例与PBL中的相当,但LPL中的T细胞明显较少。