溶菌酶催化的壳寡糖反应。

Lysozyme-catalyzed reaction of chitooligosaccharides.

作者信息

Masaki A, Fukamizo T, Otakara A, Torikata T, Hayashi K, Imoto T

出版信息

J Biochem. 1981 Aug;90(2):527-33. doi: 10.1093/oxfordjournals.jbchem.a133501.

DOI:10.1093/oxfordjournals.jbchem.a133501

Abstract

The time-courses of substrate consumption and product formation in the lysozyme-catalyzed reaction were determined with (GlcNAc)4 and (GlcNAc)5 as substrate to accumulate data suitable for the estimation of rate constants by numerical analysis. The lysozyme-catalyzed reactions were followed by TLC or HPLC. (GlcNAc)4 decomposed apparently to small oligosaccharides within 5 h, and (GlcNAc)5 decomposed within 15 min at pH 5.0 and 50 degrees C. The temperature-dependence of the rate of disappearance of the initial substrate showed a different profile from that observed with glycol chitin as substrate by the reducing power method. The order (or distribution) of the amount of product formed from (GlcNAc)5 in the reaction time-course determined by TLC differed from that determined by HPLC. The relative error in HPLC was much less than that in TLC, and the time-course determined by HPLC was thought to be of sufficient accuracy for the estimation of rate constants by computer analysis.

摘要

以（GlcNAc）4和（GlcNAc）5作为底物，测定溶菌酶催化反应中底物消耗和产物形成的时间进程，以积累适合通过数值分析估算速率常数的数据。通过薄层层析（TLC）或高效液相色谱（HPLC）追踪溶菌酶催化反应。在pH 5.0和50℃条件下，（GlcNAc）4在5小时内明显分解为小的寡糖，（GlcNAc）5在15分钟内分解。初始底物消失速率的温度依赖性呈现出与用还原力法以乙二醇几丁质作为底物时观察到的不同的曲线。通过TLC测定的反应时间进程中由（GlcNAc）5形成的产物量的顺序（或分布）与通过HPLC测定的不同。HPLC中的相对误差远小于TLC中的相对误差，并且认为通过HPLC测定的时间进程对于通过计算机分析估算速率常数具有足够的准确性。