雌二醇诱导非洲爪蟾肝脏培养物中卵黄蛋白原mRNA的积累及卵黄蛋白原的分泌。

Estradiol-induced accumulation of vitellogenin mRNA and secretion of vitellogenin in liver cultures of Xenopus.

作者信息

Felber B K, Ryffel G U, Weber R

出版信息

Mol Cell Endocrinol. 1978 Nov;12(2):151-66. doi: 10.1016/0303-7207(78)90111-9.

Abstract

Explants of male Xenopus liver maintained in a serum-free culture medium respond to stimulation by 2 X 10(-8) M 17beta-estradiol with an increasing rate of accumulation of vitellogenin mRNA, as revealed by hybridization of cDNA to the total cytoplasmic RNA extracted from the cultures. A similar response is observed for secretion of 32PO4-labeled vitellogenin into the culture medium. The in vitro response is improved in liver tissue of prestimulated animals, and by adaptation of liver explants to the culture medium prior to hormone treatment, but attains only about 10% of the in vivo response. Since essential features of the in vivo response are maintained in liver explants, organ culture appears suitable for investigating initial events of estradiol action leading to enhanced synthesis of vitellogenin.

摘要

在无血清培养基中培养的雄性非洲爪蟾肝脏外植体，对2×10⁻⁸M 17β-雌二醇的刺激有反应，卵黄蛋白原mRNA的积累速率增加，这通过将cDNA与从培养物中提取的总细胞质RNA杂交得以揭示。对于32PO4标记的卵黄蛋白原分泌到培养基中也观察到类似反应。预刺激动物的肝脏组织以及在激素处理前使肝脏外植体适应培养基，可改善体外反应，但仅达到体内反应的约10%。由于肝脏外植体保留了体内反应的基本特征，器官培养似乎适合用于研究导致卵黄蛋白原合成增强的雌二醇作用的初始事件。