Hayward M A, Brock M L, Shapiro D J
Nucleic Acids Res. 1982 Dec 20;10(24):8273-84. doi: 10.1093/nar/10.24.8273.
Estrogen induces the synthesis of vitellogenin mRNA by activating transcription of the vitellogenin genes. Quantitative inhibition of liver protein synthesis by cycloheximide does not prevent activation of vitellogenin gene transcription. The relative transcription rate of the vitellogenin genes in estrogen stimulated liver is similar in control and cycloheximide treated animals (800-1000 ppm). Selective estrogen activation of vitellogenin gene transcription therefore represents a direct effect of estrogen on vitellogenin gene transcription which can occur without any change in the cells' protein complement. Two other cellular responses to estrogen, the induction of nuclear estrogen receptor, and an increased rate of total nuclear RNA synthesis, are blocked by cycloheximide administration. Since the overall rate of vitellogenin mRNA synthesis is a function of both the selective estrogen activation of vitellogenin gene transcription which is not blocked by cycloheximide and the increased rate of total nuclear RNA synthesis which is blocked by cycloheximide, the total rate of vitellogenin mRNA synthesis is markedly reduced following cycloheximide administration.
雌激素通过激活卵黄蛋白原基因的转录来诱导卵黄蛋白原mRNA的合成。用环己酰亚胺定量抑制肝脏蛋白质合成并不能阻止卵黄蛋白原基因转录的激活。在对照动物和用环己酰亚胺处理的动物（800 - 1000 ppm）中，雌激素刺激的肝脏中卵黄蛋白原基因的相对转录速率相似。因此，雌激素对卵黄蛋白原基因转录的选择性激活代表了雌激素对卵黄蛋白原基因转录的直接作用，这种作用可以在细胞蛋白质组成没有任何变化的情况下发生。另外两种对雌激素的细胞反应，即核雌激素受体的诱导和总核RNA合成速率的增加，在用环己酰亚胺处理后被阻断。由于卵黄蛋白原mRNA合成的总体速率是由不受环己酰亚胺阻断的雌激素对卵黄蛋白原基因转录的选择性激活以及受环己酰亚胺阻断的总核RNA合成速率增加共同作用的结果，在用环己酰亚胺处理后，卵黄蛋白原mRNA合成的总速率显著降低。
