Rola-Pleszczynski M, Massé S, Sirois P, Lemaire I, Bégin R
J Immunol. 1981 Dec;127(6):2535-8.
We recently developed an animal model to study the pulmonary reactions after exposure to environmental pollutants. In this model, sheep received monthly intratracheal instillations of a suspension of varying amounts of chrysotile asbestos in saline. All animals were studied by pulmonary function tests, transbronchial biopsies, and bronchoalveolar lavage. During the 6 mo of the study, no changes were seen in the 2 former tests, but bronchoalveolar lavage yielded leukocytes that responded with enhanced proliferative activity to concanavalin A, pokeweed mitogen, and staphylococcal lysate. The response to phytohemagglutinin was poor but also enhanced in the asbestos-exposed animals. Enhanced responsiveness was dose-related, except for the highest dose used, 128 mg, which was associated with a less enhanced proliferative response or none. Our data suggest that the initial response to low-dose exposure to asbestos consists in a stimulation of lymphocyte function; the possibility that this phenomenon may be linked to the eventual fibrogenic response of asbestosis warrants further studies.
我们最近建立了一种动物模型，用于研究暴露于环境污染物后肺部的反应。在这个模型中，绵羊每月经气管内滴注不同剂量温石棉在生理盐水中的悬浮液。所有动物均通过肺功能测试、经支气管活检和支气管肺泡灌洗进行研究。在研究的6个月期间，前两项测试未发现变化，但支气管肺泡灌洗产生的白细胞对刀豆球蛋白A、商陆有丝分裂原和葡萄球菌溶素的增殖活性增强。对植物血凝素的反应较差，但在接触石棉的动物中也有所增强。除了使用的最高剂量128毫克外，增强的反应性与剂量相关，该剂量与增殖反应增强较少或无增强有关。我们的数据表明，低剂量接触石棉的初始反应是淋巴细胞功能的刺激；这种现象可能与石棉肺最终的纤维化反应有关，这一可能性值得进一步研究。
