Rossignol D P, Roschelle A J, Lennarz W J
J Supramol Struct Cell Biochem. 1981;15(4):347-58. doi: 10.1002/jsscb.1981.380150405.
We have attempted to identify a surface component of echinoderm eggs that is involved in the species-specific binding of sperm. Cell surface membranes from eggs of the sea urchins Strongylocentrotus purpuratus or Arbacia punctulata were radioiodinated, detergent-treated, and subjected to density-gradient centrifugation. In the presence of bindin, the complementary binding protein isolated from sperm, one component of the membranes sedimented to a different density. This membrane component bound species specifically to sperm that had undergone the acrosome reaction. This binding led to an inhibition of the ability of treated sperm to fertilize eggs. Exhaustive proteolytic digestion of this receptor fraction yields a high molecular weight glycopeptide that can also bind to bindin. It therefore appears that this egg surface membrane fraction contains a functionally intact, species-specific receptor for sperm.
我们试图鉴定棘皮动物卵子的一种表面成分，该成分参与精子的物种特异性结合。对紫球海胆或斑点海胆卵子的细胞表面膜进行放射性碘化、去污剂处理，然后进行密度梯度离心。在存在从精子中分离出的互补结合蛋白结合素的情况下，膜的一种成分沉降到不同的密度。这种膜成分与经历了顶体反应的精子进行物种特异性结合。这种结合导致处理过的精子使卵子受精的能力受到抑制。对该受体部分进行彻底的蛋白水解消化会产生一种也能与结合素结合的高分子量糖肽。因此，看来这种卵子表面膜部分含有一种功能完整的、物种特异性的精子受体。
