Langeveld J P, Veerkamp J H, Duyf C M, Monnens L H
Kidney Int. 1981 Jul;20(1):104-14. doi: 10.1038/ki.1981.111.
Pairs of glomerular and tubular basement membrane preparations were obtained from kidneys of individuals of premature age up to 80 years. The purity of the preparations was established with light and electron microscopy and by estimating the total phosphorus content. The amino acid and carbohydrate composition was determined and statistically evaluated for 33 to 38 preparations divided over five age groups. Comparison of glomerular and tubular basement membranes from the same kidneys showed that regardless of age glomerular basement membranes contain more 3-hydroxyproline and more of the heteropolysaccharide constituents neuraminic acids and mannose. These differences cannot be ascribed solely to the presence of a minor amount of interstitial collagen. The chemical composition of the two types of basement membranes changes with age. For the first few years after birth the contents of glycine and total imino acids increase and those of collagen-nonspecific amino acids decrease, whereas constituents of the heteropolysaccharide units do not change markedly. These results suggest that proportions of collagenous and noncollagenous peptide moieties gradually change with age in both glomerular and tubular basement membranes. In addition, the extent of hydroxylation of proline and lysine increases significantly with age, reaching an adult level for glomerular basement membranes after 4 to 7 months of age and for tubular basement membranes during late childhood. The contents of glucose and galactose rise with age to an extent comparable with that of hydroxylysine. The age-related changes in basement membrane composition may influence functional properties of these extracellular renal structures.
从年龄不足80岁的早产儿肾脏中获取成对的肾小球和肾小管基底膜制剂。通过光学显微镜和电子显微镜以及估算总磷含量来确定制剂的纯度。对分为五个年龄组的33至38份制剂测定其氨基酸和碳水化合物组成并进行统计学评估。对取自同一肾脏的肾小球和肾小管基底膜进行比较，结果表明，无论年龄大小，肾小球基底膜都含有更多的3 - 羟脯氨酸以及更多的杂多糖成分神经氨酸和甘露糖。这些差异不能仅仅归因于少量间质胶原的存在。两种类型的基底膜的化学成分随年龄而变化。出生后的头几年，甘氨酸和总亚氨基酸的含量增加，而胶原非特异性氨基酸的含量减少，而异多糖单元的成分没有明显变化。这些结果表明，肾小球和肾小管基底膜中胶原和非胶原肽部分的比例随年龄逐渐变化。此外，脯氨酸和赖氨酸的羟基化程度随年龄显著增加，肾小球基底膜在4至7个月龄后达到成人水平，肾小管基底膜在儿童晚期达到成人水平。葡萄糖和半乳糖的含量随年龄增加，增加程度与羟赖氨酸相当。基底膜组成的年龄相关变化可能会影响这些肾脏细胞外结构的功能特性。
