Cederholm B, Wieslander J, Bygren P, Heinegård D
Proc Natl Acad Sci U S A. 1986 Aug;83(16):6151-5. doi: 10.1073/pnas.83.16.6151.
It has recently been shown that patients with IgA nephropathy have circulating IgA antibodies against extracts of human glomerular basement membrane. The present study extends those observations and demonstrates by using ELISA and immunoblotting techniques that patients with IgA nephropathy have circulating IgA antibodies against collagen IV alpha chains. The antigenicity of the alpha chains could be destroyed by digestion with collagenase, which indicates that the antigenic site(s) is located on the triple helical part of collagen IV. Furthermore, it was shown by inhibition tests that the IgA antibodies are directed against epitopes also present in collagen I and II isolated after pepsin digestion.
最近研究表明，IgA肾病患者体内存在针对人肾小球基底膜提取物的循环IgA抗体。本研究扩展了这些观察结果，并通过酶联免疫吸附测定（ELISA）和免疫印迹技术证明，IgA肾病患者体内存在针对IV型胶原α链的循环IgA抗体。α链的抗原性可被胶原酶消化破坏，这表明抗原位点位于IV型胶原的三螺旋部分。此外，抑制试验表明，IgA抗体所针对的表位也存在于经胃蛋白酶消化后分离出的I型和II型胶原中。
