Myohara K, Hiwatashi K
Genetics. 1978 Oct;90(2):227-41. doi: 10.1093/genetics/90.2.227.
Cells of Paramecium caudatum, syngen 3 usually become sexually mature about 50 fissions after conjugation. In order to study the genetic mechanisms that control fission-department expression of maturity, an attempt was made to obtain early mature mutants by treatment with N-methyl-N'-nitro-N-nitrosoguanidine. A new cytoplasmic marker, erythromycin resistance, was used to eliminate nonconjugant and macronuclear regeneration clones. Twenty early mature clones were obtained from five different mutagenized cultures. Three of them were genetically analyzed by crosses to wild-type stocks. The results show all three mutants to be controlled by incompletely dominant genes, i.e., the homozygotes became mature 20-25 fissions and the heterozygotes 15 fissions earlier than the wild-type clones. At least two different loci are suggested for the early maturity.
尾草履虫同宗配合3型的细胞在接合后通常经过约50次分裂达到性成熟。为了研究控制成熟分裂表达的遗传机制，人们尝试用N-甲基-N'-硝基-N-亚硝基胍处理来获得早熟突变体。一种新的细胞质标记，即红霉素抗性，被用来排除非接合体和大核再生克隆。从五个不同的诱变培养物中获得了20个早熟克隆。其中三个通过与野生型品系杂交进行了遗传分析。结果表明，所有三个突变体均由不完全显性基因控制，即纯合子比野生型克隆早熟20-25次分裂，杂合子早熟15次分裂。早熟现象至少涉及两个不同的基因座。
